Low molecular weight heparin (LMWH) protect vascular endothelial cells in hypoxia-reoxygenation condition / 重庆医科大学学报

ABSTRACT

Objective:To investigate whether LMWH can protect the function of umbilical cells in hypoxia and hypoxia-reoxygenation conditions.Methods:Human umbilical vein endothelial cells,—ECV304,were planted into 6-well dishes,then cultivated respectively in three conditionsnormal,hypoxia and hypoxia-reoxygenation conditions.Cells were grown for 1 hour in hypoxia condition;reoxygenated for 6 or 24 hours after 1 hour hypoxia,respectively.Cells in hypoxia and hypoxia-reoxygenation conditions were divided into two subgroupsLMWH group (5IU/ml) and control group.At each end of incubation time the medium was removed from each well.NO and MDA were measured by chromatometry.ET were measured by radioimmunoassay.Results:There was a decrease in the biosythesis and release of NO,while an increase of MDA and ET in hypoxia and hypoxia-reoxygenation conditions.The effect in hypoxia-reoxygenation condition was more remarkable than in hypoxia condition.But in both conditions LMWH could inhibit the productions of MDA and ET,increase the productions of NO.Conclusion:In hypoxia and hypoxia-reoxygenation conditions,LMWH can inhibit the production of MDA,and protect endothelial cells from oxygen free radicals.