Determination of ferulic acid and rhein in Shengfaling Tincture by HPCE / 中成药

ABSTRACT

AIM: To determine the content of ferulic acid and rhein in Shengfaling Tincture(Radix Angelicae Sinensis,Flos Carthami,Radix Polygoni Multiflori,ect.). METHODS:HPCE was used.The best condition was obtained with a fuse dsilca capillary tube (60 cm?75 ?m,effective length of 53 cm),30 mmol?L -1 sodium tetraborate electrophoretic buffer(pH 8.2),at a constant voltage of 12 kV and temperature at 25 ?C .The UV detection wavelength was at 313 nm and caffeic acid was adopted as internal standard. RESULTS:The calibration curves was linear in the range of 2.4-12 ?g?mL -1 for ferulic acid(r=0.999 7) and 1.6-8 ?g?mL -1 for rhein (r= 0.999 6 ),respectively.The average recovery of ferulic acid was 99.06% and that of rhein was 98.94%. CONCLUSION: This method is simple,quick and sensitive.