Effect of Electroacupuncture on Synaptic Transmission in Hippocampal Dentate Gyrus of Rats with Cerebral Ischemic Damage / 广州中医药大学学报

ABSTRACT

[ Objective ] To investigate the effect of electroacupuncture ( EA) on synaptic transmission in hippocampal dentate gyrus of rats with cerebral ischemic damage. [Methods] Sixty Wistar rats were enrolled into this study. Among them, 30 were given high-frequency stimulation (HFS) and the other 30 were not. Then the HFS rats were randomized into sham-operation group (group A), model group (group B) and acupuncture group (group C); so did the non-HFS rats. Bilateral carotid arteries were exposed but not blocked in Group A, while were blocked by traction with silk thread for modeling in group B. Group C were treated with EA before and after modeling. Effects of EA on synaptic transmission in hippocampal dentate gyrus of non-HFS rats were evaluated with in-vivo long-term potential ( LTP) recorded by electrophysiological technique. Effects of EA on LTP in hippocampal dentate gyrus of HFS rats were observed after LTP was induced by HFS. [Results] In non-HFS rats, no significant changes of magnitude of population spike (PS) occurred within 120 min in group A; magnitude of PS in group B decreased at 10th min after modeling ( P