Study of Kanglaite Injection Combined with Chemotherapeutic Drugs in Inhibiting the Growth of Transplanted Human Lung Cancer A549 in Nude Mice / 中药新药与临床药理

ABSTRACT

Objective To observe the attenuated effect of Kanglaite Injecti on (KI) combined with chemotherapeutic drugs on transplanted human lung cancer A54 9 in nude mice. Methods Ninety- six nude mice were transplanted subcutaneousl y with human lung cancer A549; 7 days later, the mice were randomized into 16 gr oups(n=6) and were given antitumor drugs according the regimen. Other 12 normal mice were used as controls. KI groups were given KI 1.25, 2.5 and 5.0 g/kg respe ctively and were administered (iv) continuously for 10 times on 7th day of trans plantation; cisplatin (DDP, 1.5 mg/mg or 3 mg/kg) and Gemzar (30 mg/kg or 60 mg/ kg) were given one dose by intraperitoneal injection on 10th day of transplantat ion; Gemzar was given again 4h after medication of DDP. Taxotere (25 mg/kg) was given one dose by intraperitoneal injection on 7th day of transplantation. The m ice were killed to examine the body weight, tumor weight and tumor- inhibiting rate. Results KI 1.25, 2.5 and 5.0 g/kg respectively combined with DDP 3mg/kg and Gemzar 60 mg/kg had a better inhibitory effect on the growth of human lung c ancer A549 than KI, DDP and Gemzar alone. KI in three doses respectively combine d with DDP 1.5 mg/kg and Gemzar 30 mg/kg not only had a better inhibitory effect than those of KI, DDP and Gemzar alone, but also promoted the antitumor actions . The tumor- inhibitory rate of KI in three doses respectively combined with Ta xotere (25 mg/kg) was higher than KI and Taxotere alone. Conclusion KI combine d with DDP and Gemzar or with Taxotere can shorten the tumor mass and has tumor - inhibitory and attenuated effect.