IsoIation and 2-DE analysis of mitochondria of cultured hepatoma cells / 重庆医科大学学报
Journal of Chongqing Medical University
; (12)1986.
Article
in Zh
| WPRIM
| ID: wpr-573522
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WPRO
ABSTRACT
Objective:To make the isolation and 2-DE analysis of mitochondria,in order to prepare the following proteomic study on mitochondria of hepatoma-cell. Methods:Mitochondria was separated from cell homogenate by means of density gradient centrifugation,and 2-DE was conducted to examine the protein profile of mitochondrial preparations.Results:The increase in purity of mitochondria was found to be 12 times by density gradient centrifugation.Mitochondrial proteins were displayed well in the 2-DE pattern after purification.Conclusion:The isolation procedure is practicable,which provides a basis for the following proteomic study on mitochondria.
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WPRIM
Language:
Zh
Journal:
Journal of Chongqing Medical University
Year:
1986
Type:
Article