Effect of silencing mdrl gene by RNA interference with adriamycin on proliferation of SKOV_3/ADM / 重庆医科大学学报

ABSTRACT

Objective:To investigate the effect of RNA interference targeting mdrl gene with ADM on ovarian cancer resistant strain SKOV 3/ADM.Methods:The expression of mdrlmRNA after transfected pshRNA-mdrl was detected by RT-PCR;MTT test and clone forming test were applied to measure the inhibition combined with adriamycin on proliferation of SKOV 3/ADM strain.Results:These pshRNA-mdrls inhibited the expression of mdrlmRNA and proliferation of SKOV 3/ADM obviously when combined with adriamycin.Conclusion:The short hairpin RNA recombinant plasmid targeting mdrl gene combined with adriamycin inhibits the proliferation of SKOV 3/ADM and increases the sensitivity to chemotherapy.