The tffects of two tyrosine kinase inhibitors on the proliferation of colon cancer cell / 重庆医科大学学报
Journal of Chongqing Medical University
;
(12)1986.
Article
in Chinese
| WPRIM
| ID: wpr-573560
ABSTRACT
Objective:
To investigate the effects of Genistein and Tyrphostin AG1478 on the proliferation of colon cancer cell HT-29 and its possible mechanism.Methods:
The effect of two tyrosine kinase inhibitors (TKIs) on HT-29 cell lines was studied by using MTT assay.Immunocytochemical staining was performed with PCNA polyclonal antibody in HT-29 cells.Western blot assay was used to detect the expression of ERK-1 and pERK.Results:
Both Genistein and Tyrphostin AG1478 significantly inhibit the growth of HT-29 cells and showed concentration dependence.Two tyrosine kinase inhibitors suppressed PCNA expression.Western blot revealed that Genistein and Tyrphostin not inhibit ERK-1 expression but inhibit phosphorylation of ERK.Conclusion:
Tyrosine kinase inhibitors Genistein and Tyrphostin AG1478 can inhibit the growth of HT-29 colon cancer cells,the anti-proliferation mechanism may be related to down-regulate the expression of pERK.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Journal of Chongqing Medical University
Year:
1986
Type:
Article
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