Determination of luteolin and isorhamnetin in Duyiwei Capsules by HPLC / 中成药
Chinese Traditional Patent Medicine
;
(12)1992.
Article
in Chinese
| WPRIM
| ID: wpr-573707
ABSTRACT
AIM:
To establish the method for determination of luteolin and isorhamnetin in Duyiwei Capsules (Lamiophl mis rotata(Benth.)Kudo).METHODS:
Yilite-C_(18) column was used.The mobile phase was composed of methanol-water(50∶50),pH was adjusted to 3.0 with H_3PO_4.The flow rate was 0.8 mL?min~(-1) and detection wave-length was set at 360 nm.RESULTS:
The linear range of Luteolin was 7.072-35.360 ?g?mL~(-1),(r=(0.999 7)) and the linear range of isorhamnetin was 1.658-8.290 ?g?mL~(-1)(r=0.999 2). The average recovery and RSD of luteolin were 96.3% and 2.7%,respectively. The average recovery and RSD of isorhamnetin were 97.3% and 2.1%,respectively.CONCLUSION:
This method is sensitive,accurate and can be used for the quality study of Duyiwei Capsules.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Chinese Traditional Patent Medicine
Year:
1992
Type:
Article
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