The research on bifidobacterium inhibits inflammatory response in murine experimental colitis / 中华消化杂志

ABSTRACT

Objective To investigate the effect of bifidobacterium (Bif) supplementation on acute inflammatory response in murine dextran sulfate sodium(DSS)-induced colitis, and its possible mechanism. Methods Mice were divided into four groups control,DSS, salfasalazine (SASP) and Bif. The mice in groups DSS, SASP and Bif were fed with 5% DSS(w/v) solution for 7 days to induce colitis, and disease activity index (DAI) was calculated every day. The mice in group SASP were fed with SASP every day during inducing colitis, and the mice in Bif group were given Bif by oral gavage from 7 days before the experiment to the end of experiment. The expression of TNF-?、NF-?B P65 and myeloperoxidase (MPO) in inflamed colon of each group was measured at the end of experiment. Results The mice in groups SASP and Bif showed a lower DAI than those in group DSS since the forth day to the end of experiment. There were lower level of TNF-? and MPO in murine inflammatory colon, and lower NF-?B P65 expression in nuclei of inflammatory cells in groups SASP and Bif than those in control at the end of experiment. Conclusions Treatment with Bif can effectively inhibit proinflammatory cytokine secretion and NF-?B activation in inflammatory cells, and decrease colonic inflammatory response in DSS induced colitis.