Determination of Emodin and Chrysophanol Contents in Qingre Anchuang Tablets by HPLC / 中药新药与临床药理

ABSTRACT

Objective To determine the contents of emodin a nd chrysophanol in Qingre Anchuang tablets(QAT)by HPLC.Method The C 18 column was applied.Methanol -0.1%p hosphoric acid solution(90∶10)served as the mobile phase.The wavelength of detection was at 440nm.Results Emodin and chrysophanol in QAT were c an be isolated com-pletely,and the range of linear correlation was 0.0543～0.3258?g for emodin(r=0.99997)and 0.1048～0.6288?g for chrysophanol(r=0.99999).The average recovery rate of emodin and chrysophanol was 96.87%(RSD =1.98%,n=6)and 96.22%(RSD =1.88%,n=6)respectively.Conclusion The method was accurate,reliable and with good reproducibility.It could be used for quality control of QAT.