Content Determination of Trigonelline in Radix Mirabilis by RP-HPLC / 中药新药与临床药理
Article
in Zh
| WPRIM
| ID: wpr-573752
Responsible library:
WPRO
ABSTRACT
Objective To determine the content of trigonelline in Radix Mirabilis.Methods A RP -HPLC method was established.The chromatographic c olumn was Inertsil NH 2 Columns(250?4.6mm,5?m.).The mobile phase was acetonitrile -water(80∶20).The flow rate was 0.8mL?min -1 and the detection wavelength was at 265nm,and the column temperature was 30℃.Results The calibration curve was linear in t he range of 0.1872~0.9360?g .The aver-age recovery for trigonelline was 99.6%and RSD was 1.76%(n=6).Conclusion The method is simple and effec-tive and can be used to determine the c ontent of trigonelline in Radix Mirabilis.
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Index:
WPRIM
Language:
Zh
Journal:
Traditional Chinese Drug Research & Clinical Pharmacology
Year:
1993
Type:
Article