Thymosin ?_4 raises survival rate and down-regulatesinflammatory mediators in septic shock mice / 重庆医科大学学报

ABSTRACT

Objective:To explore the mechanism of action of thymosin ? 4 (T? 4) in septic shock by observing the 72h survival rate of septic shock mice treated with thymosin ? 4 and exploring the changes of TNF-? and IL-1?.Methods:LPS(lipopolysaccharide) was intraperitoneally injected into clean Kunming mice to establish the animal model.①The T? 4,between normal and septic shock mice were determined.②The survival rate of septic shock mice was studied by intraperitoneal injection of T? 4.Animals were divided into four groups of 30 and each received LPS(25mg/kg)intraperitoneal injection.Group Ⅰ was LPS control group.GroupⅡ,Ⅲ and Ⅳ were individually injected with T? 4 at 0h,0h,2h;0h,2h,4h post LPS through caudal vein.③TNF-? and IL-1? were examined in septic shock mice by ELISA.Results:T? 4 was obviously decreased in the blood of septic shock mice.T? 4 could raise the 72h survival rate of septic shock mice and down-regulate inflammatory mediators.Conclusion:The optimal protective methods in the mouse appears to be 5mg/kg T? 4 given immediately following(0h) and at 2h and 4h successively post LPS.T? 4 down-regulates the inflammatory mediators,which may be the mechanism of T? 4 in curing the septic shock.