Influence on humoral immunity in mice by goat placenta immunoregulatory factor / 重庆医科大学学报

ABSTRACT

Objective:To study the humoral immunity effect of goat placenta immunoregulatory factor(GPIF).Methods:Basing on BALB/c mice,the study was carried out by determining the proliferation of B lymphocyte in mice spleen with MTT method,the level of IgM with PFC test,and the level of IgG secreted by mice B cell in vivo and in vitro with ELISA test.Results:GPIF could effeciently increase the level of antibody in vivo.Direct experiment on splenic B lymphocyte,GPIF could also improve the level of antibody in vitro and enable mice lymphocyte induced by LPS to proliferate efficiently.Conclusion:GPIF conld enhance humoral immunity function and recover the ability of humoral immunity in mice with immunodeficiency.