Expression of neural stem cell-specific molecules cultured in vitro and induced mononuclear cells derived from human cord blood / 重庆医科大学学报

ABSTRACT

Objective:To investigate the configuration changes of human cord blood monocytes (HCMNCs)under specific culture conditions and the expression of NSCs marker under induction.Methods:HCMNCs were prepared from normal placenta after full term normal delivery. Observe the shape by microscope and reverse transcriptase polymerase chain reaction (RT-PCR) for nestin and musashi-1 antigen were performed to confirm nestin and musashi-1 antigen expression in HCMNCs.Results:RT-PCR demonstrated small amount of nestin expressed in HCMNCs but no musashi-1 antigen before induction, and both showed the strongest expression in 48h,then faded away gradually. The uncultivated MNCs were small and round.After culturing,the cells became larger,some cells change their shape into rhombic.Conclusion:The newly HCMNCs isolated by centrifugation over Lymphoprep densitgradient expressed nestin mRNA in low level and no musashi-1 show up,after induction,the former and musashi-1 increased intermittently and then decreased with the time of culturing.