Dynamic changes of FasL/Fas apoptosis pathway in islets of NOD mice / 重庆医科大学学报

ABSTRACT

Objective:To investigate expression of cytokines and apoptosis factors in islets of female NOD mice at different weeks of age.Methods:54 female NOD mice were randomly divided into three groups(C1,C2,C3)and each group consisted of 18 mice.The mice were killed respectively at 4th,15th,30th week (of onset of diabetes),and their panreases were removed.The levels of expression of cytokines and apoptosis factors mRNA of the pancreas were detected by RT-PCR.The expressions of cytokines and apoptosis factors protein were measured by using immunohistochemical method.Insulitis scores were observed by HE staining.Results:Expressions of Fas and IFN-? mRNA in pancreas were stronger in C3 vs C1 or C2,in C2 vs C1(P0.05).Expressions of Fas and CPP32 were stronger in islet cells in C2 and C3 vs C1(P