Pharmacokinetic of saikosaponin d in Wuling Capsules in mouse / 中成药

ABSTRACT

AIM To investigate the pharmacokinetic characters of saikosaponin d in Wuling Capsules in mouse. METHODS Wuling Capsules were given by irrigated (ig) administered to mouse, the blood plasma was collected at different time and the sample solution was prepared by extract adding amine sulfate and the mix fluid of acetonitrile-methyl alcohol (4∶ 1), the content of saikosaponin d was detected by RP-HPLC. The pharmacokinetic parameter was fitted by SIP7 software. RESULTS After ig administered the Wuling Capsules (5.5 g/kg) to mouse, the blood plasma concentration-time course fitted well to one compartment model with the 1st order absorption. The pharmacokinetic parameters of the first absoption in mouse was as following Ka=2.34_ 1/h ,Ke=0.08_ 1/h ,T_ max =1.45_h,C_ max =83.81_ ?g/ml ,T_ 1/2Ke =7.9 h; and that of the second absorption was as following Ka=1.32_ 1/h ,Ke=0.08_ 1/h ,T_ max =6.48h,C_ Max =119.63_ ?g/ml , T_ 1/2 Ke =7.76 h. CONCLUSION The boipharmaceutics characters of saikosaponin d of Wuling Capsules were absorption slow and elimination was also slow.