NEUROPROTECTION WITH AMINOGUANIDINE TREATMENT AFTER BRAIN INJURY IN RATS / 解剖学报

ABSTRACT

Objective To investigate the neuroprotective effect of inducible nitric oxide synthase(iNOS)inhibitor,aminoguanidine(AG),after brain injury in rats. Methods Using frontal sharp cortex injury model,SD rats were randomly divided into AG treat group and NS control group.Biochemical assay,RT-PCR,immunohistochemistry and TUNEL method were used to determine NO content,iNOSmRNA,iNOS positive cells and apoptotic cells respectively.The experimental results were compared in the two groups. Results NO content and the expression of iNOSmRNA and iNOS positive cells in traumatized cortex decreased significantly in AG treat group than that in NS control group.The number of apoptotic cells also reduced correspondently in AG treat group.Conclusion AG could selectively inhibit the expression of iNOS,then reduce NO content,thus reduce apoptotic cells and produce protective effect on cells in injuryed area.