Determination of 1-deoxynojirimycin from mulberry resources and analysis of their bioactivities / 中草药

ABSTRACT

Objective To determine 1-deoxynojirimycin(DNJ) from mulberry resources and analyse their bioactivities.Methods Determination of DNJ by derivatization with 9-fluorenylmethyl chloroformate(FMOC-Cl) and followed by reversed-phase high-performance liquid chromatography(RP-HPLC) and a fluorescence detector;the inhibitory curves on ?glucosidase at the enzymatic level and the activities to(?-)glucosidase in vitro were observed in the everted sac experiment.Results A reliable quantitative method suitable for assays of DNJ from mulberry resources has been developed.The inhibitory curves of the extracts on ?-glucosidase were parallel to those of DNJ.The extracts can inhibit hydrolyzation of starch and absorption of glucose.Conclusion The medicinal parts from mulberry resources can be used for diabetic therapy as ?-glucosidase inhibitor.