Role of human leukocyte antigen-G small interference RNA in a choriocarcinoma cell line / 中华妇产科杂志

ABSTRACT

Objective To study the role of human leukocyte antigen-G (HLA-G) in the carcinogenesis and development of choriocarcinoma. Methods We designed and synthesized a double strand small interference RNA (siRNA) of HLA-G, then transfected it into a HLA-G overexpressed choriocarcinoma cell line, JEG-3 by lipofectine; HLA-G mRNA level was detected by real time RT-PCR; HLA-G protein level was detected by western blot. The living cells of JEG-3 were counted under microscope after transfection by siRNA. Results Double strand siRNA of HLA-G effectively downregulated the mRNA level and the protein level of HLA-G. The mRNA levels by Ct value at different concentration (1.0, 2.5, 5.0 ?g/L) were 20.67?0.02, 21.37?0.03,21.43?0.02, respectively. They were significantly different compared with the control groups which were 20.33?0.01, 20.37?0.02,20.40?0.03, respectively ( P