The comparative study of growth inhibition effect of arsenic trioxide on human breast cancer MCF-7 and MCF-7/ADR cell / 重庆医科大学学报

ABSTRACT

Objective:To explore the difference between the growth inhibition effect of arsenic trioxide(As_2O_3)on the human breast cancer MCF-7 and multidrug resistance MCF-7/ADR cell line.Methods:MCF-7 and MCF-7/ADR cell was cultured with 0.5?mol/L～16?mol/L arsenic trioxide in vitro.MTT test was adopted to detect the growth suppression effect of As_2O_3 in the two cell lines.Apoptosis morphology was studied with light microscope and electron microscope.Apoptosis ratio was analysed with flow cytometry.Results:As_2O_3 could inhibit the proliferation of the MCF-7 and MCF-7/ADR cell line,especially that of 2?mol/L～16?mol/L,and the effect was dose-and-time dependent.The IC_(50) at 96h was 3.0?mol/L for MCF-7 and 12.8?mol/L for MCF-7/ADR.Cell morphology and flow cytometry revealed the growth suppression was caused mainly by apoptosis rather than necrosis;however,to obtain the same inhibitory level,MCF-7/ADR cell required higher concentration of As2O3 and longer processing time.Conclusion:As_2O_3 can inhibit the proliferation in MCF-7/ADR cell as well as MCF-7 cell,though MCF-7/ADR cell is tolerant to As_2O_3,but the tolerance is at a low level.