Interventional effect of polygonum cuspidatum sieb.et Zucc.on NOS in atherosclerosis rabbits in vivo / 重庆医科大学学报

ABSTRACT

Objective:To study the interventional effect of polygonum cuspidatum sieb.et Zucc.on NOS system in vivo.Methods:Thirty-six Japanese rabbits were randomized into 6 groupsNC Group (fed normal control diet),HC group (fed atherogenic diet),SHZ group,MHZ group,LHZ group(fed atherogenic diet and three different doses of polygonum cuspidatum sieb.et Zucc.separately)and L-Arg group (fed atherogenic diet and L-arginine). At the different feeding time,the following contents were surveyed1.serum NO,NOS,plasma ET;2.EDD and NEDD of external iliac artery in vivo;3.the area of AS plaque in thoracic aorta;4.NOS activity and the expression of iNOS mRNA and eNOS mRNA on the wall of the abdominal artery.Results:In group HC,EDD and NEDD of external iliac arteries were damaged at 1 week and 6 weeks,separately;the levels of serum NO,plasma ET and activity of NOS were elevated significantly;AS lesion was serious;the activity of NOS and expression of iNOS mRNA on the medial layer of the artery wall were much higher,the activity of NOS and expression of eNOS mRNA on endothelial cells were much lower.Except NEDD,polygonum cuspidatum sieb.et Zucc.could not only adjust the activities and expressions of disordered NOS systems on AS vessels,but also improve AS lesion in a dose-effect manner.Large dose of polygonum cuspidatum sieb.et Zucc.had the strongest effect.Conclusion:Polygonum cuspidatum sieb.et Zucc.can improve the function of the disordered NOS and lessen AS lesion on AS vessels in a dose-dependent manner.