A role of sodium selenite in regulating expression of Cyclooxygenase-2(COX-2) mRNA in rat mesangial cells / 重庆医科大学学报

ABSTRACT

Objective:To observe the effect of sodium selenite on expression of Cyclooxygenase-2 (COX-2) mRNA in rat mesangial cells (RGMC), and to study the mechanism of sodium selenite in anti-diabetic nephropathy.Methods:RGMC was incubated with high glucose,advanced glycosylation end products (AGE),high insulin and H_2O_2 respectively;simultaneously,RGMC pre-treated with sodium selenite was incubated with above factors.The expression of COX-2 mRNA was detected and compared.Results:The four factors increased the expression of COX-2 mRNA independently;sodium selenite inhibited the expression of COX-2 mRNA by the four factors distinctly.Conclusion:Sodium selenite suppresses the expression of COX-2 mRNA in RGMC, which suggests that sodium selenite may play a significant role in the prevention of diabetic nephropathy by inhibition of the expression of COX-2 mRNA in RGMC.