NS-398, a selective cyclooxygenase-2 inhibitor, can down-regulate the expression of P-glycoprotein in the gastric cancer cell / 中华消化杂志
Chinese Journal of Digestion
; (12)2001.
Article
in Zh
| WPRIM
| ID: wpr-574133
Responsible library:
WPRO
ABSTRACT
Objective To investigate the effect of NS-398, a selective cyclooxygenase-2 (COX-2) inhibitor, on the expression of P-glycoprotein(P-gp) in a gastric cancer cell line SGC-7901. Methods SGC-7901 cells were treated with NS-398 in different concentrations (0, 10 ?mol/L and 100 ?mol/L) respectively. Prostaglandin E2(PGE2) was detected by ELISA. Twenty four hs and 48 hs later, the expression of mdr1 mRNA were detected by RT-PCR. P-gp protein expression in SGC-7901 cells was detected by immunocytochemical technique after NS-398 treatment.Results NS-398 can inhibit the expression of PGE2 in a dose-dependent manner(P
Full text:
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Index:
WPRIM
Language:
Zh
Journal:
Chinese Journal of Digestion
Year:
2001
Type:
Article