HPLC fingerprint of ultramicro decoction piece of Fructus gardeniae / 中成药

ABSTRACT

AIM: To determine the HPLC fingerprint of ultramicro decoction piece of Fructus gardeniae. METHODS: Chromatographic conditions were as follows column Hypersil BDS C_ 18 (4.6 mm?200 mm,5 ?m), mobile phase acetonitrile-water (10∶90), column tempemture 25 ℃,flow rate 1 mL?min -1 , wavelength 238 nm, and inject volume 10 ?L. RESULTS: The fingerprint of ultramicro Fructus gardeniae was established and 7 common peaks in the fingerprint were indicated. CONCLUSION: It can nearly completely reflect intrinsic quality of ultramicro decoction piece of Fructus gardenian.