The mechanism of ~(131)I-GM-CSF inducing apoptosis of HL-60/ADM cells / 重庆医科大学学报
Journal of Chongqing Medical University
;
(12)2003.
Article
in Chinese
| WPRIM
| ID: wpr-574248
ABSTRACT
Objective:
To investigate the mechanism of ~(131)I-GM-CSF inducing apoptosis of HL-60/ADM cells.Methods:
An in vitro radioimmunotherapy(RAIT) model was employed.Data were collected from transmission electrion microscopy,flow cytometric analysis and immunocytochemistry assay.Results:
After exposure to ~(131)I-GM-CSF,HL-60/ADM cells were induced to apoptosis.The cell mitochondrial transmembrane potential(MTP) reduced and HL-60/ADM cells appeared the classical apoptotic morphology.The Bax was up regulated,while Bcl-2 and Bcl-xl was decreased.Conclusion:
~(131)I-GM-CSF can induce HL-60/ADM apoptosis through opening the mitochondrial permeability transition pore and reducing MTP,which suggests that ~(131)I-GM-CSF may be available in therapy of AML.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Journal of Chongqing Medical University
Year:
2003
Type:
Article
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