Study on Extraction Method for Assaying Jinyou Tankeqing Tablets / 中药新药与临床药理

ABSTRACT

Objective To optimize the extraction method for assaying Jinyou Tankeqing Tablets(JTT).Methods With the total flavonoids content as the index,Orthogonal Experimental Design was used to optimize the extraction condition for assaying JTT.Results The optimized ultrasonic extraction method was as follows adding 0.2 mL?mg-1 powder to 95 % ethanol,extracting for 30 min;the optimized Sohlex extraction method was adding 1.2 mL?mg-1 powder to 95 % ethanol,extracting for 2 h,the former method being better than the later.Conclusion The optimized extraction method for assaying JTT is adding 0.2 mL?mg-1 powder to 95 % ethanol and extracting for 30 min with ultrasonic wave.