Determination of Bilirubin in Xihuang Capsules by HPLC / 中药新药与临床药理
Traditional Chinese Drug Research & Clinical Pharmacology
;
(6)2000.
Article
in Chinese
| WPRIM
| ID: wpr-574280
ABSTRACT
Objective To establish a method for the determination of bilirubin in Xihuang capsules.Methods HPLC method was applied and the chromatographic conditions were as follows Hypersil BDS C18 column(150 mm ? 4.6 mm,5 ?m)with methanol-chloroform-1 % phosphoric acid(84︰9︰7)as the mobile phase.The flow rate was 1.0 mL ? min-1 with the detected wavelength at 450 nm and the column temperature at 25 ℃.Results The calibration curve for bilirubin was linear in the range of 0.01 ~ 0.07 ?g.The average recovery was 98.07 %(RSD = 1.05 %).Conclusion This method is simple,accurate,specific and could be used for the quality control of bilirubin in Xihuang capsules.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Traditional Chinese Drug Research & Clinical Pharmacology
Year:
2000
Type:
Article
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