Huzhang Decoction inhibits TNF-? and apoptosis in liver injury induced by CCl_4 in rats / 中成药

ABSTRACT

AIM: To observe the effects of Huzhang Decoction(HZD)(Rhizoma Polyqonicuspidati,Radix Sanguisorbae,Herba Saururi,Rhizoma curcumae,etc) on tumor necrosis factor ?(TNF-?)and apoptosis in liver injury induced by CCl_4 in rats. METHODS: Wistar rats were treated with HZD(1.0g/kg or 2.0g/kg) after 3days by administration of CCl_4(1 ml/kg,po).The levels of TNF-? in serum were measured by enzyme-linked immunosorbent assay(ELISA).The apoptosis cells in liver tissue were tested by TdT-mediated digoxin-dUTP nick end labeling(TUNEL),and the positive expression of bcl-2 or bax by strept avidin-biotin complex(SABC). RESULTS: After being treated by HZD the serum level of TNF-? and apoptosis index was decreased obviously in rat with liver injury induced by CCl_4,and the ratio of bcl-2/bax was increased markedly(compared with liver injury group,P