OBSERVATION BY SCANNING ELECTRON MICROSCOPY ON RABBIT KIDNEY AFTER THE RELEASE OF CHRONIC URETERAL OBSTRUCTION / 解剖学报

ABSTRACT

Objective To explore the mechanism of imperfect recovery of the renal function after release of chronic ureteral obstruction. Methods Adult New Zealand rabbits(n=36) were randomly divided into normal control group(n=3),obstructive control group(n=3) and experimental group(n=30).The latter two groups were subjected to chronic unilateral ureteral obstruction.Two weeks later,3 rabbits were sacrificed and the others underwent ureterostomy,which were sacrificed separately following 12 hours,24 hours,3 days,7 days,14 days.The renal papilla and cortex were examined by scanning electron microscopy. Results Shortly after the release of ureteral obstruction,massive loss of brush border and ballooning of epithelial cells were noted in proximal tubules.Distal tubules and collecting tubules also showed severe ballooning of epithelial cells and normal struction were not seen.Two weeks later,many tubular components became patent with normal epithelial lining.Some of them,however,were deteriorated further in spite of the release of obstruction.Thus there was a marked tubular heterogeneity in recovery.Conclusion Further deterioration of some tubules might be one of the important pathologic basis for imperfect recovery of renal function after the release of chronic ureteral obstruction.