Study on the anti-inflammatory mechanism of diammonium glycyrrhizinate in acetic acid-induced colitis of rats / 中华消化杂志

ABSTRACT

Objective To evaluate the role of diammonium glycyrrhizinate(DG) in experimental colitis and to probe into its underlying mechanisms.Methods Tirty female Spragur-Dawley rats were randomized into treatment group,acetic acid control and normal control groups(10 rats for each group).Colitis in treatment group and acetic acid control was induced by administrating acetic acid rectally.The rats in treatment group were treated with DG.Disease activity index(DAI) and histological damage as well as myeloperoxidase(MPO) activation were observed.The nuclear factor-?B(NF-?B) activity,the(expressi)on of tumor necrosis factor-?(TNF-?) and intercellular adhesion molecule-1(ICAM-1) in colon tissues were detected by immunohistochemistry method.Results In treatment group,acetic acid control(and normal) control,the DAI were 3.5?0.6,7.1?0.8 and 0.5?0.4 respectively;the histological(damage) scores were 3.5?0.9,6.1?1.0 and 1.0?0.5 respectively;MPO activation were 0.72?0.08,2.02?0.10 and 0.22?0.04 respectively;the positive percentages of TNF-? were 35.2?8.2,62.5(?10.1) and 7.9?5.7 respectively,the positive percentages of ICAM-1 were 34.3?8.2,60.2?8.3 and 9.1?3.4 respectively;the positive percentages of NF-?B were 23.3?9.2,44.5?8.9 and 9.6?4.4(respectively).(Compared) with the acetic acid control group,the DAI and histological damage scores as well as MPO(activation) of colon tissues in treatment group were significantly improved(P