Effects of Polysaccharide L01 from Laminaria on the Expression and Secretion of PAI- 1 in Cultured Human Umbilical Vein Endothelial Cell / 中药新药与临床药理
Traditional Chinese Drug Research & Clinical Pharmacology
;
(6)1993.
Article
in Chinese
| WPRIM
| ID: wpr-574639
ABSTRACT
Obejective To study the effects of polysaccharide L01 from Laminaria on the expression and secretion of plasminogen activator inhibitor- 1 (PAI- 1) stimulated by adrenaline in cultured human umbilical vein endothelial cells (HUVEC). Methods With adrenaline added, human umbilical vein endothelial cell line ECV- 304 was exposed to different concentrations of polysaccharide L01 of Laminaria and cultured for 24, 48, and 72 hours respectively in vitro. The levels of PAI- 1 in cultured supernants were measured with enzyme- linked immunosorbent assay( ELISA) .Reverse transcriptase chain reaction( RT- PCR) was used to detect the expression of PAI- 1 messenger RNA( mRNA) in HUVEC, and the related products were examined by density scan and half- quantitative analysis after electrophoresis. Results Stimulated by 10 ? g/mL adrenaline for 48h and 72 h, the levels of PAI- 1 in cultured supernants increased significantly (P
Full text:
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Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Traditional Chinese Drug Research & Clinical Pharmacology
Year:
1993
Type:
Article
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