Regenerated plantlet from salt-tolerant callus of Glycyrrhiza uralensis / 中草药

ABSTRACT

Objective To develop the technique of artificial cultivation of Glycyrrhiza uralensis on salina by exploring regenerated plantlet from the salt-tolerant callus.Methods The experiments of dedifferentiation were successful in inducing cotyledon sections and embryonic axle sections from sterilized shoot,as explants,on salty media.The induced callus was to be multiplied and adventitious buds tobe produced when the salt concentration of medium increased gradually.Then the buds were rooting and a lot of plantlet occurring.Results Dedifferentiation of cotyledon sections and embryonic axle sections was preferable on MS+BA 1.5 mg/L+2.4-D 1.2 mg/L+NaCl 100 mg/L.Callus was hazel and just a little translucent.Growth of callus became weaker and weaker after increasing concentration of NaCl to 250 mg/L,the color got darker,and much callus died.A lot of adventitious buds came into being on MS+BA(0.5 mg/L+)KT 1.0 mg/L+NAA 1.0 mg/L+NaCl 200 mg/L after inoculating the callus on non-salty media for multiplying twice.It proved that the salt-tolerant character of the buds was induced by reason of genetics.Decreasing temperature(18—22 ℃) at natural light and adding an appropriate quantity of GA_3 in medium were helpful for the formation of strong sprouts.The rooting rate was 66.26% with rooting 55 adventitious buds among 83,and medium of 1/2 MS+IAA 1.0 mg/L+NAA 1.0 mg/L+NaCl 200 mg/L adapted to root. Conclusion A lot of salt-tolerant regenerated plantlets of G.uralensis occur after inducing the salt-tolerant callus to redifferentiate for getting the regenerated plantlet.