Protection and mechanism of notoginsenoside-Rg_1 on focal cerebral ischemia-reperfusion rats / 中草药

ABSTRACT

Objective To explore the effects of notoginsenoside-Rg_1 on brain-derived neurotrophic factor(BDNF) protein in cerebrum cortex of MCAO/R injury,as well as to investigate whether notoginsenoside-Rg_1 can up-regulate the protein content of BDNF of the positive neurons or the amount of the po-sitive neurons.Methods Adult male SD rats(60) were randomly divided into model group,notoginsenoside-Rg_1 high,middle,and low dose(200,100,50 mg/kg) groups,and the positive control(Nimodipine,1 mg/kg) group.All drugs were given once a day by ip till the mouse was killed.The focal cerebral(ischemia-)reperfusion model was made with thread-occluded method.Their frozen brain tissue were sliced(into) section of 12 ?m thickness.The four rats were randomly taken from each groups to be treated as specimens after surgical handle in 1,3,and 7 d.The slices were according to the immunohistochemical ABC techniques.The protein content of BDNF of the positive neurons and the amount of the positive neurons in cerebrum cortex of rat were observed and counted by HPIAS—1000 analytic system,and the nervous deficit symptoms after the cerebral ischemia were observed.Results Comparing with the model group,all notoginsenoside-Rg_1 treated groups obviously improved some nervous deficit symptoms of cerebral ischemia-reperfusion rats,and increased the protein content of BDNF and the amount of the positive neurons in the cerebrum cortex of model rats(P