Establishment of experimental model of chronic alcoholic fatty liver in rats / 重庆医科大学学报

ABSTRACT

Objective: To establish an experimental animal model of chromic alcoholic fatty liver.Methods: Fifty three Wister rats were divided into four groups control group and model groups(lower dose ethanol group?medium dose ethanol group and higher dose ethanol group).The rats in model groups were induced by ethanol intragastric infusion of 40%(v/v)ethanol orally [7g/(kg?d) for lower group?8 g/(kg?d) for medium group and 9 g/(kg?d) for higher group],two times a day for the first 4 weeks.Ethanol intragastric infusion of 50%(v/v)ethanol orally [8 g/(kg?d) for lower group,9 g/(kg?d) for medium group and 10 g/(kg?d) for higher group],two times a day for the second 4 weeks.Then,the liver was examined by Ultrasonography.The pathologic changes were observed and the amount of ALT?AST??-GT in serum were detected at the end of the eighth week.Results: At the end of eighth week,serum AST and ?-GT levels were increased significantly in model groups.The typical fatty liver pathologic changes of chronic alcoholism were observed in rats in medium and higher dose model groups.Conclusions: Experimental model of chronic alcoholic fatty liver can be established in rats with ethanol intragastric infusion.