A study on apoptosis in isolated pancreatic islet cells induced cytokines and its mecanism / 重庆医科大学学报
Journal of Chongqing Medical University
;
(12)1987.
Article
in Chinese
| WPRIM
| ID: wpr-574840
ABSTRACT
Objective:
To investigate the apoptosis of isolated pancreatic islet cells and insulin secretion induced by combination of IL-1?,TNF and IFN-?,and to investigate the mechanism of the change.Methods:
Isolated pancreatic islet cells from Wistar rat were incubated in monolayer in vitro.NO production,NOS activity,insulin secretion,the protein expression of bcl-xl and bax,apoptotic rate of islet cells and DNA fragmentation in pancreatic islet cells incubated with combination of IL-1?,TNF and IFN-? were measured.Results:
Combination of IL-1?,TNF and IFN-? induced a significant increase in apoptotic percentage of pancreatic islet cells,NO production and NOS activity,as well as DNA ladder appearance,while a decrease in insulin content.Up-regulation of the protein expression of bax and down-regulation of the protein expression of bcl-xl were also induced(P
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Journal of Chongqing Medical University
Year:
1987
Type:
Article
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