Applied study of large TCM compound recipe fingerprint / 中成药

ABSTRACT

AIM: Provided the integrated method for large TCM recipe chemical study with the establishment of Aozhou formulae,imcluding 20 herbs. METHODS: With the comparison of Aozhou formulae and its herbs fingerprint chromatogram,it extracted fingerprint characters from chromatogram after the method establishment.According to the test results,the retention data,on-line UV and MS spectral were collected as the Aozhou formulae fingerprint database.Combined with three character classified data layers named as herb jurisdiction,multi herb jurisdiction and no herb jurisdiction,the Aozhou formulae was identified by database mentioned above with the 10 batches results. RESULTS: It presented that formulae identification by character data was more accurate than by LC/UV or LC/MS data merely.According to the comparison with median, the deviation were ?2.7%,?10.5%,(?11.2%) identified by character, LC/UV and LC/MS respectively. CONCLUSION: From the method described in this article,it could be more accurate and sensitive to reflect quality and substance characteristic by the comparison of characters extracted from compound recipe fingerprint chromatogram.And also it provided method to study the substance basement of large TCM formulae.