LIVER FIBROSIS WAS IMPROVED BY TRANSPLANTATION WITH HUMAN MESENCHYMAL STEM CELLS IN CARBON ETRACHLORIDE-INDUCED HEPATOCIRRHOSIS RATS / 解剖学报

ABSTRACT

Objective To study the effect of transplanted with human mesenchymal stem cells(MSCs) on the rat liver fibrosis in carbon tetrachloride(CCl-4)-induced hepatocirrhosis rats. Methods Wistar rats were divided into control(n=8) and hepatocirrhosis groups,and 24 hepatocirrhosis rats were made by subcutaneous injection with 60% CCl-4-peanut oil for 7 weeks,named 7-week hepatocirrhosis group(n=8).Other 7 weeks hepatocirrhosis rats were subdivided into MSC control group(n=8) and MSC transplanted group(n=8).After identification to MSCs with FACS calibur flow cytometry,MSCs were injected into the superior mesenteric vein for 3 weeks in MSC transplanted group.All of rats were killed at the beginning of 11-week.The clinical blood biochemical parameters from the inferior vena cava were examined.The survival of human MSCs in liver was observed by immunohistochemistry reaction of anti-hEGF-R.The degree of liver cirrhosis was evaluated by stains of Sirius red. Results Plasma ALP,ALB and CHO were abnormal to varying degree in 7-week hepatocirrhosis,MSC control and MSC transplanted groups.In 7 weeks hepatocirrhosis rats,a large amount of hyperplastic collagenous fiber was presented in liver tissue and the frame of the pseudolobuli was formed.These were similar to MSC control group.Only in MSC transplanted group,a few human EGF-R(+) cells were observed in livers.The amount of hyperplastic collagenous fiber in MSC transplanted group was significantly lower than those of the 7-week hepatocirrhosis and MSC control gruops.Conclusion Liver fibrosis could be improved obviously in hepatocirrhosis rats transplanted with human MSCs.These experimental results could probably provide an experimental evidence of treatment of liver cirrhosis.