SELECTION OF ADULT HAIR FOLLICLE STEM CELLS AND THE EFFECTS OF ?-CATENIN / 解剖学报

ABSTRACT

Objective To establish a convenient method for the selection of hair follicle stem cells and study the effects of ?-catenin on the regulation of proliferation and differentiation of hair follicle stem cells.Methods Based on the strong specific adherent ability of stem cells,hair follicle cells were incubated in the plates coated with human collagen before use.Hair follicle stem cell's marker K19 and clone forming efficency were used to identify the stem cells.The expression of ?-catenin was tested by immunocytochemistry and in situ hybridization.Results After adherence to collagen for 20min,the number of Kl9 positive cells among quickly-adhered cells increased 1.6 times more than unselected cells and clone forming efficiency increased 3.5 times.The expression of ?-catenin protein increased in the nuclear of quickly-adhered cells.Conclution The selection method of human hair follicle stem cells by quick-adherence to collagen was established for the first time.?-catenin was involved in the regulation of hair follicle stem cells'proliferation and differentiation.