Protective Effect of Taurine on Diaphragm in Diabetic Rats / 广州中医药大学学报

ABSTRACT

[Objective] To observe protective effect of taurine, a main component of Calculus Bovis and Scorpio, on diaphragm in diabetic rats. [Methods] Twenty-four SD rats were equally randomized into normal control group, model group and taurine group. Except the normal control group, the other rats were given intraperitoneal injection of streptozotocin 50mg/kg to induce diabetic models. Taurine group was fed with 10g/L water solution of taurine, and the other two groups with water for 4 weeks. After treatment, the rats were executed and the isolated diaphragm strips were prepared. Single contraction (SC) , maximum titanic tension, contraction time, half relaxation time, force-frequency curve and fatigue index (FI) of the diaphragm strips were examined. The contents of blood sugar, superoxide dismustase (SOD) and malondialdehyde (MDA) in the diaphragm were detected and the diaphragm ultrastructure was also observed. [Results] SC, maximum titanic tension and fatigue index were decreased, contraction time and half relaxation time prolonged, diaphragm tension in force-frequency curve decreased, SOD activity reduced and MDA content increased in the model group (P