Fingerprint in chemical compatibility of Anti-aging Compound Recipe / 中草药

ABSTRACT

Objective To study the variance of fingerprint peaks in the processes of Anti-aging Compound Recipe formation,compatibility,and preparation.Methods Anti-aging Compound Recipe and its herbs′ fingerprint chromatogram were studied and validated by HPLC/UV and LC/MS which were used in chemical compatibility.Results Based on the 18 code peaks from compound recipe fingerprint and compared with herb fingerprints,18 code peaks of compound recipe were under the assignment of herb source,that was,No.1,2 from Ganoderma No.11L,21L;No.4,5 from Fructus Ligustri Lucidi No.1N,4N;No.3,8,9,10,11,12,13,14, 18 from Fructus Schisandrae.No.1W,4W,12W,14W,16W,18W,22W,26W,31W;and No.6,7,15,16,17 were no assignment.The assignment resulted the peaks of herb source could be divided into several fingerprint regions,as anti-aging formulae,6—14 min mainly from Ganoderma, 20—30 min from Fructus Ligustri Lucidi,after 40 min from Fructus Schisandrde.All of them enhanced what the study on chemical compatibility of Anti-aging Compound Recipe was aimed at.Conclusion The two actions have appeared during mixing the crude Chinese medicinal materials into a formula recipe,one is no influence among the coexisted raw materials,while the other is with influence each other.In Anti-aging Compound Recipe the three co-decocted raw materials and their sequence put into are considered.Final results present that Fructus Schisandrae adding sequence could influence the quality of compound recipe which illustrated the compatibility among herbs and determined the variance of herbs during the preparation of compound recipe.And the results also provide a feasible way to study the compatibility and quality of compound recipe.