Determination of N,N-Dimethylformamide in oligosaccharide of medicine by Gas Chromatography / 中成药

ABSTRACT

AIM: The quantitative determination of N,N-dimethylf in olgosaccharide was carried out. METHODS: Gas Chromatographic was employed. RESULTS: Experimental results showed that a good separation of DMF with other contents of sample was achieved on FFAP(50 m?0.32 mm) column,and the separation degree was more than 1.5.The linear range was 3.58～0.000358 mg/mL with r=0.999 9 and the relative standard deviation was 1.52%(n=5).and the limit of detection was 1.0 mg/mL.The recovery of the method was in the range of 95.7%～98.4%. CONCLUSION: The method is simple,rapid,accurate and precise.