Cloning and sequencing of human apM1 gene / 重庆医科大学学报

ABSTRACT

Objective: To clone human apM1 gene for the study molecular mechanism of the function of adiponectin.Methods: RT-PCR method was used to isolate,from the total RNA of human adipose tissue,cDNA fragment encoding adiponectin,which was linked into pGEM-T Vector,then amplified,and cloned.The sequence was analyzed to identify the recombinant plasmids.Results: The result of sequencing indicated that the sequence of the cloned cDNA was identical to that of the corresponding parts of the mRNA for human apM1 in GeneBank except 2 nucleotides.Conclusion: Human apM1 cDNA gene was successfully cloned.