Determination of Berberine Hydrochloride in Siji Sanhuang Capsules / 中药新药与临床药理
Traditional Chinese Drug Research & Clinical Pharmacology
;
(6)1993.
Article
in Chinese
| WPRIM
| ID: wpr-575563
ABSTRACT
Objective To establish a method for the determination of berberine hydrochloride in Siji Sanhuang capsules(SSC).Methods The RP-HPLC method was performed on Agilent Hypersil ODS column(4.6 mm?150 mm,5 ?m).Acetonitrile and 0.05 mol/L NaH2PO4 water(adjust pH to 3 with H3PO4)were used as the mobile phase(30 ∶70),detection wavelength was at 270 nm,the flow rate was 1.0 mL/min and the column temperature was at 25 ℃.Results In the range of 0.094 to 0.470 ?g,berberine hydrochloride had a good linearity with chromatograph peaks area(r=0.998 3),the average recovery ratio was 98.5 %(n=5),and RSD=2.3 %.Conclusion This method is simple,effective and with a higher sensibility and stability.The method can be used to control the quality of SSC effectively.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Traditional Chinese Drug Research & Clinical Pharmacology
Year:
1993
Type:
Article
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