Protective effect of proteasome inhibitor MG-132 on myocardial reperfusion injury in rats / 重庆医科大学学报

ABSTRACT

Objective: To investigate the effect of proteasome inhibitor MG-132 on myocardial infarction size and biochemical marker levels in rat myocardium after reperfusion injury.Methods: Adult Sprague-Dawley rats were randomly divided into 4 groups left anterior descending(LAD) coronary artery ligation for 30 minutes without treatment(I30min),LAD ligation for 30 minutes with subsequent 6 hours reperfusion without treatment(I30min/R6h),LAD ligation for 30 minutes with subsequent 6 hours reperfusion with treatment(I30min/R6h+I30min/R6h) and Sham(Sham30min).After LAD was ligated for 25 min,at 5 min before reperfusion,MG-132(0.75mg/kg)or sterile saline was given to all the treatment group/sham rats,the myocardial infarct size,levels of serum creatine kinases(CK),CK muscle-brain fraction(CK-MB),and troponin I(TnI),were measured.Results: Compared with Sham group,the myocardial infarct size and the ratio of the volume of myocardial infarct over the total volume of left ventricular wall,as well as the levels of serum CK,CK-MB,TnI were significantly increased in I30mingroup(P