The distribution and expression of recombinant M.smegmatis in mice receiving intranasal immunization / 重庆医科大学学报

ABSTRACT

Objective: To observe the distribution and expression of recombinant Mycobacterium smegmatis carrying the plasmid encoding human granulysin(GLS) and murine IL-12 by intranasal immunization in mice.Methods: BALB/C mice were intranasally immunized with recombinant M.smegmatis carrying the plasmid encoding green fluorescent protein(GFP).The distribution of GFP and recombinant Mycobacterium smegmatis in the lungs and spleens was detected 14 days after the first immunization.BALB/C mice were intranasally immunized three times with recombinant M.smegmatis carrying the plasmid encoding GLS and IL-12.28 days after the first immunization,the expression of GLS in tissue,levels of IL-12 in serum and SIgA in BALF(bronchoalveolar lavage fluid)were detected with immunohistochemistry and ELISA respectively.Results: The distribution of GFP and recombinant Mycobacterium smegmatis in lung and spleen was detected.The expression of GLS,the increased of IL-12 in serum and the specific SIgA in BALF were found.Conclusion: The distribution of recombinant Mycobacterium smegmatis carrying the plasmid encoding of GFP in lung and spleen is detected.The specific anti-bacterium SIgA is generated.GLS and IL-12 are expressed in BALB/C mice after intranasal immunization with recombinant Mycobacterium smegmatis carrying the plasmid encoding GLS and IL-12.The results lay the foundation for new vaccine study.