Amplification of 5′cDNA end of human augmenter of liver regeneration by 5′-RACE technique and analysis of sequence / 重庆医科大学学报
Journal of Chongqing Medical University
;
(12)1986.
Article
in Chinese
| WPRIM
| ID: wpr-575723
ABSTRACT
Objective:
To amplify the 5′ cDNA end of human augmenter of liver regeneration(hALR) by 5′-RACE technique and to analyze the sequence.Methods:
5′ cDNA end of hALR was rapidly amplified by following the instructions of 5′-RACE kit(Takara).The acquired sequence was analyzed by bioinformatic tool.Results:
5′-RACE product with 487bp was successfully obtained,which included extending sequence with 275bp.Homology analysis by BLAST showed highly homologous with human citron kinase.Conclusions:
5′-RACE method can be used to amplify the 5′ end of partially known cDNA sequence.Citron kinase can interact with hALR.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Journal of Chongqing Medical University
Year:
1986
Type:
Article
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