ISSR Identification of genetic diversity in Aconitum carmichaeli / 中草药

ABSTRACT

Objective ISSR Identification of genetic diversity in Aconitum carmichaeli by ISSR marker technique. Methods Genetic diversity between Jiangyou Radix Aconiti Lateralis Preparata and nine wild A. carmichaeli populations was determined by ISSR technique. Results Eight primers were selected to produce highly reproducible ISSR bands. Among 98 amplified bands, 68 showed polymorphism, the percentage of polymorphic bands (PPB) reached to 69.39%. Observed number of alleles (Na), effective number of alleles (Ne), Nei′s gene diversity index (H), and Shannon information index (I) were 1.693 9, 1.371 5, 0.230 8, and 0.353 0, respectively. A DNA profile was discovered with a single primer, ISSR 855, in which each of ten tested populations had its unique patterns and was distinguished from each other. Conclusion ISSR Method is suitable for DNA fingerprinting, identification , and genetic diversity analysis of A. carmichaeli.