Prepartion of reference substance of flavonoid in Desmodium styracifolium by RP-HPLC / 中成药
Chinese Traditional Patent Medicine
;
(12)1992.
Article
in Chinese
| WPRIM
| ID: wpr-575896
ABSTRACT
AIM:
To develop an RP-HPLC method for preparing the reference substanc of vicenin-2 in Desmodium styracifolium.METHODS:
Ethanol-extract of desmodium styracifolium was isolated and purified by RP-HPLC combining solvent extraction with column chromatography and recrystalliztion.The purity and content of vicenin-2 were identified by HPLC.RESULTS:
The flvonoids were completely separated under this chromatographic condition.The purity of the reference substance was 99.0% or above.CONCLUSION:
The method is simple,accurate,better on repeatability,and effective to yield high-purity product.It can be used as reference substance for the research of herbal medicine.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Chinese Traditional Patent Medicine
Year:
1992
Type:
Article
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