The effect of ginsenoside-Rg1 on the expression of Bcl-2 and Bax in rabbit lens epithelial cell induced by oxidative injuries / 重庆医科大学学报

ABSTRACT

Objective:To investigate the effect of Rg1 on the expression of apoptosis related genes of LEC induced by experimental oxidative injuries.Methods:All fresh transparent lenses of rabbit except those in the control group were bathed in a culture medium with 300?mol/L hydrogen peroxide(H2O2)to establish cataract models in vitro.Meanwhile Rg1 were added to the culture medium and incubated for 24 hours.The anterior capsules were used in the following study.The expressions of apoptosis related genes Bcl-2 and Bax protein were studied by immunohisto chemical techniques.Results:Bcl-2 and Bax protein were expressed in LEC of normal rabbit lens.Expression of Bcl-2 protein was much stronger than that of Bax protein.The expression of Bcl 2 protein was down-regulated and that of Bax protein was up-regulated in H2O2 group when compared with the control group,so the ratio of Bcl-2/Bax was lower.Rg1 up-regulated the expression of Bcl-2,and down-regulated that of Bax protein;the ratio of Bcl-2/Bax was higher.There were significant differences in the range of ratio changes compared to that of the taurine group.Conclusion:Rg1 can up-regulate the expression of Bcl-2,and down-regulate that of Bax protein,to increase the ratio of Bcl-2/Bax;while the expression of Bcl-2 protein was down-regulated and that of Bax protein was up-regulated in the H2O2 group.The molecular mechanisms of both H2O2 inducing apoptosis of LEC and natural anti-oxidants restraining apoptosis of LEC may be related to the expressions of apoptosis-related gens Bcl-2 and Bax.