Research on the effects and side effects of the over-dose chemotherapy to VX2 hepatocarcinoma rabbits with mdr1 gene transferring bone marrow transplantation / 重庆医科大学学报

ABSTRACT

Objective:In the study,multidrug resistance gene 1(mdr1) was transferred into the bone marrow mononuclear cells which were autotransplanted into rabbits with VX2 hepatocarcinoma.In chemotherapy experiment with adriamycin,the bone marrow protection of mdr1 gene,curative effects and side effects to heart of over dosage chemotherapy were observed.Methods: The bone marrow mononuclear cells transferred with mdr1 gene were autotransplanted into rabbits with VX2 hepatocarcinoma.After chemotherapy with adriamycin,the peripheral white blood cells count,the growth and metastasis of VX2 hepatocarcinoma and cardiac function were detected.Meanwhile,the bone marrow protection of mdr1 gene,the curative effects to VX2 hepatocarcinoma and lesions to heart of over-dosage chemotherapy were evaluated.Results:The mdr1 gene was implanted into the bone marrow after the mdr1-transferred bone marrow mononuclear cells had been autotransplanted into rabbits with VX2 hepatocarcinoma.After over-dose chemotherapy with adriamycin,the rabbits in mdr1-transferring group could be survival after treated with 3-fold dosage chemotherapy,and the white blood cell count in peripheral blood was(4.26?1.03)?109/L.Meanwhile,the tumor cells were killed effectively,and the survival time were improved compare to that of control group(P